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Demi-Fraser Broth (DFB)
Tryptone 5g
Peptone 5g
Beef Extract 5g
Yeast Extract 5g
Sodium Chloride 20g
Disodium Phosphate 9.6g
Monopotassium Phosphate 1.35g
Esculin 1g
Lithium Chloride 3g
Nalidixic Acid 0.01g
Acriflavin 0.012g
Ferric Ammonium Citrate 0.01g
Final pH: 7.2 ± 0.2 at 25°C
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