Triple Sugar Iron Agar (TSI)
Description
The Triple Sugar Iron (TSI) test is a microbiological test named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. It is often used to differentiate enteric bacteria including Salmonella and Shigella
Formula:
Enzymatic Digest of Casein 5g
Enzymatic Digest of Animal Tissue 5g
Sodium Thiosulfate 0.3g
Sucrose 10g
Sodium Chloride 5g
Ferric Ammonium Citrate 0.2g
Phenol Red 0.025
Agar 13.5g
Final pH: 7.3 ± 0.2 at 25°C
Resources:
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